Creepcast_scientist

mailnam@mail.com

?>

2025-03-13 02:17:28

His-tag protein purification woes

If its not binding to nickel its not going to bind to other metals lil bro. Maybe the N-term is internal

1741833022
Damn getting lil bro’d it’s been a rough day. I’ve gotten other proteins perfectly purified this is just my special little boy that’s not working

1741833098
At least you're not a regard who makes a K to C mutation and runs it through a Q column, assuming it will still ft, and doesn't check gels before torching a week of work in ethanol.

1741834124
Is all life pain big bro?

1741834252