Creepcast_scientist

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2025-03-13 02:17:28

His-tag protein purification woes

If its not binding to nickel its not going to bind to other metals lil bro. Maybe the N-term is internal

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Damn getting lil bro’d it’s been a rough day. I’ve gotten other proteins perfectly purified this is just my special little boy that’s not working

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At least you're not a regard who makes a K to C mutation and runs it through a Q column, assuming it will still ft, and doesn't check gels before torching a week of work in ethanol.

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Is all life pain big bro?

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