Gel Screening help :,)
Hey fellow labrats! I just finished doing a maxiprep. The DNA yield I got was pretty decent and I decided to run a digestion to screen for the plasmid. I was supposed to get two bands one at approx 6.0kb and the other around 2.0kb. The plasmid total is 8kb. However, the band I got was slightly lower than 2.0kb and the 6.0kb band is practically nonexistent. Now I’m at a loss at what is going on. Please can someone help shed some light on what is going on. Any advice would be much appreciated
Hey fellow labrats! I just finished doing a maxiprep. The DNA yield I got was pretty decent and I decided to run a digestion to screen for the plasmid. I was supposed to get two bands one at approx 6.0kb and the other around 2.0kb. The plasmid total is 8kb. However, the band I got was slightly lower than 2.0kb and the 6.0kb band is practically nonexistent. Now I’m at a loss at what is going on. Please can someone help shed some light on what is going on. Any advice would be much appreciated