Negative control Gibson
Hi,
I pcrd fragment 1 (one type of plasmid) and pcrd fragment 2 (another type of plasmid), now o want to assemble them by Gibson.
In the -ve control reactions, I want to include both fragments individually as negative controls, how could I do that pls?
What are other things I should think about for controls?
For the assembly I use neb Gibson mmix
Positive control is neb Gibson positive control fragments
Thanks